Version
- Product Details
- Antigen Details
- Documentation
- Reviews
- Related Protocols
- Related Products
- Related FAQs
- Other Formats
- Certificate of Analysis
- Clone
- Poly5160 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Tumor necrosis factor-α, Cachectin, Necrosin, Macrophage cytotoxic factor (MCF), Differentiation inducing factor (DIF), TNFSF-2
- Isotype
- Goat Polyclonal IgG
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Compare all formats
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 516003 | 50 µg | 300⬠| ||||
Description
TNF-α is secreted by macrophages, monocytes, neutrophils, T-cells (principally CD4+), and NK-cells. Many transformed cell lines also secrete TNF-α. Monomeric mouse TNF-α is 156 amino acid protein (N-glycosylated) with a reported molecular weight of 17.5 kD protein. TNF-α forms multimeric complexes; stable trimers are most common in solution. A 26 kD membrane form of TNF-α has also been described. TNF-α binding to surface receptors elicits a wide array of biologic activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorraghic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils.
Product Details
Technical data sheet
Product Details
- Verified Reactivity
- Mouse, Rat
- Antibody Type
- Polyclonal
- Host Species
- Goat
- Immunogen
- Recombinant mouse TNF-α
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
- ELISA Detection - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 0.25 - 1.0 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of TNF-α recombinant protein ranging from 500 to 7.8 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
- Additional Product Notes
ELISA Detection:The biotinylated Poly5160 antibody is useful as a detection antibody for a sandwich ELISA when used in conjunction with purified TN3-19.12 antibody (Cat. Nos. 506102 & 506106) as the capture antibody for detecting mouse and rat TNF-α. The biotinylated Poly5160 antibody can also be used as a detection antibody in a sandwich ELISA to detect mouse TNF-α when used in conjunction with purified MP6-XT22 (Cat. Nos. 506301 & 506302) and recombinant mouse TNF-α(Cat. No. 575209) as the standard.
Note:For testing mouse TNF-αin serum, plasma or supernatant, BioLegend's LEGEND MAX™ Kits (Cat. Nos. 430907 & 430908) are specially developed and recommended.- Product Citations
- RRID
- AB_2561411 (BioLegend Cat. No. 516003)
Antigen Details
- Structure
- TNF superfamily; dimer/trimer; 18-150 kD (Mammalian).
- Distribution
-
Activated monocytes, neutrophils, macrophages, T cells, B cells, NK cells, LAK cells.
- Function
- Type II integral membrane protein processed by TACE for secretion; upregulated by interferons, IL-2, GM-CSF, substance P, bradykinin, PAF, immune complexes, cyclooxygenase; downregulated by IL-6, TGF-β, vitamin D3, prostaglandin E2, PAF antagonists.
- Interaction
- Monocytes, neutrophils, macrophages, T cells, fibroblasts, endothelial cells, osteoclasts, adipocytes, astroglia, microglia.
- Ligand/Receptor
- TNFRSF1A (TNF-R1, CD120a, TNFR-p60 Type β, p55); TNFRSF1B (TNF-R2, CD120b, TNFR-p80 Type A, p75).
- Cell Type
- B cells, Macrophages, Monocytes, Neutrophils, NK cells, T cells
- Biology Area
- Immunology
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Beutler B, et al. 1988. Annu. Rev. Biochem. 57:505.
3. Beutler B, et al. 1989. Annu. Rev. Immunol. 7:625.
4. Tracey K, et al. 1993. Crit. Care Med. 21:S415. - Gene ID
- 21926 View all products for this Gene ID
- Specificity (DOES NOT SHOW ON TDS):
- TNF-alpha
- Specificity Alt (DOES NOT SHOW ON TDS):
- TNF-α
- App Abbreviation (DOES NOT SHOW ON TDS):
- ELISA Detection
- UniProt
- View information about TNF-alpha on UniProt.org
Documentation
- Technical data sheet
- Certificate of Analysis
- Safety Data Sheet
Reviews
Review This Product
Related Protocols
- Active Protocols: Sandwich ELISA - Video
- Sandwich ELISA Protocol
Related Products
| Description | Clone | Applications |
|---|
Pathways
- 14-3-3 Induced Intracellular Signaling
- Cellular Apoptosis Pathway
- Chemokine Receptor Biology
- Cytokine Network
- Gamma-Delta T cells
- IL-10 Pathway
- ILK Signaling
- Immunologic Networks 2011
- Mechanisims of Cell Death
- Neuroinflammation
- Th17 Biology
- TH2 Pathway (Humoral Immune Response)
- TNF Superfamily Pathway
Pages
- Quality Control
- Streptavidin Conjugates
- MojoSort™ Magnetic Cell Separation
- Interleukin Receptors
- Chemokines and Chemokine Receptors
Related FAQs
- How many biotin molecules are per antibody structure?
- We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Other Formats
View All TNF-α Reagents Request Custom Conjugation
| Description | Clone | Applications |
|---|---|---|
| Biotin anti-mouse/rat TNF-α | Poly5160 | ELISA Detection |
| LEAF™ Purified anti-mouse/rat TNF-α | Poly5160 | Neut,WB |
Certificate of Analysis
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For Research Use Only. Not for diagnostic or therapeutic use.
This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for userâs intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.
BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.
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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Biotin anti-mouse/rat TNF-α
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LEAF™ Purified anti-mouse/rat TNF-α
Recombinant mouse TNF-α (Cat. No. 575206) (black circles) cy... Whole cell extracts (15 µg protein) from RAW264.7 cells that...
