Ultra-LEAF Purified anti-human IL-6 Antibody, IL-6, MQ2-13A5 (2024)

Version: 1 Revision Date: 06/19/2019

  • Product Details
  • Antigen Details
  • Documentation
  • Reviews
  • Related Protocols
  • Related Products
  • Related FAQs
  • Other Formats
  • Certificate of Analysis

Clone
MQ2-13A5 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-6, B cell stimulating factor-2 (BSF-2), Cytotoxic T cell differentiation factor (CDF), Hepatocyte stimulating factor (HSF), Hybridoma/plasmacytoma growth factor (HPGF)
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
Ultra-LEAF Purified anti-human IL-6 Antibody, IL-6, MQ2-13A5 (1)
  • Ultra-LEAF Purified anti-human IL-6 Antibody, IL-6, MQ2-13A5 (2)

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Cat # Size Price Quantity Check Availability Save
501125 100 µg £125
501126 1 mg £444

Description

IL-6 is a potent lymphoid cell growth factor that stimulates the growth and survival of certain B cells and T cells. IL-6 plays a role in host defense, acute phase reactions, immune response, and hematopoiesis. IL-6 is expressed by T cells, B cells, monocytes, fibroblasts, hepatocytes, endothelial cells, and keratinocytes.

Product Details

Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
COS-7- expressed, recombinant human IL-6
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application
ELISA Capture - Quality testedCyTOF® - VerifiedELISPOT Capture, Neut, ICFC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISPOT capture antibody, a concentration range of 2.0 - 8.0 µg/ml is recommended. For ELISA capture applications, a concentration range of 0.5 - 2.0 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-6 recombinant protein ranging from 1000 to 8 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA or ELISPOT Capture1-3,7: The purified MQ2-13A5 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MQ2-39C3 antibody (Cat. No. 501202) as the detecting antibody.

Neutralization1-3,5,6: The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human IL-6 bioactivity (Cat. No. 501110). The MQ2-13A5 antibody can neutralize the bioactivity of natural or recombinant IL-6.

Additional applications (for the relevant formats) include: intracellular flow cytometry10.

Application References

(PubMed link indicates BioLegend citation)

  1. Abrams J, et al. 1992. Immunol. Rev. 127:5.
  2. Abrams JS. 2001. Curr. Protoc. Immunol. Unit 6.20.
  3. Gaines Das R, et al. 1993. J. Immunol. Methods 160:147.
  4. Enriquez J, et al. 2002. Adv. Perit Dial. 18:177.
  5. Zou JP, et al. 1999. J. Immunol. 162:4882.
  6. Wyant TL, et al. 1999. Infect. Immun. 67:1338.
  7. Lesmeister MJ, et al. 2005. Reprod. Biol. Endocrinol. 3:74.
  8. Terasaka Y, et al. 2010. Invest. Ophthalmol. Vis. Sci. 51:2441 PubMed
  9. Girndt M, et al. 1998. J. Am. Soc. Nephrol. 9:1689.
Product Citations
  1. OConnor RA, et al. 2021. OncoImmunology. 10(1):1940675. PubMed
  2. Yang CY, et al. 2021. Stem Cell Res Ther. 193:12. PubMed
  3. Cui L, et al. 2022. J Biomed Sci. 29:101. PubMed
RRID
AB_2810625 (BioLegend Cat. No. 501125)
AB_2810626 (BioLegend Cat. No. 501126)

Antigen Details

Structure
Neuropoietic cytokine family; 26 kD (Mammalian)
Bioactivity
Hematopoiesis; antigen-specific immune responses, inflammatory reactions; acute phase reaction
Cell Sources
T cells, B cells, macrophages, bone marrow stromal cells, fibroblasts, keratinocytes, mesangium, astrocytes, endothelial cells
Cell Targets
T cells, B cells, hepatocytes, cholinergic neurons
Receptors
Heterodimer IL-6Rα (CD126)/IL-6Rβ (CD130; gp130)
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Hirano T. 1998. Int. Rev. Immunol. 16:249.
3. Patterson P. 1992. Curr. Opin. Neurobiol. 2:94.
4. van Oers M, et al. 1993. Ann. Hematol. 66:219.

Regulation
Upregulated by IL-1, TNF, PDGF, IFN-β, TNF-α, NGF, and IL-17; downregulated by glucocorticoids IL-4 and TGF-β
Gene ID
3569 View all products for this Gene ID
UniProt
View information about IL-6 on UniProt.org

Documentation

Reviews

Review This Product

Related Protocols

  • Active Protocols: Sandwich ELISA - Video
  • Sandwich ELISA Protocol

Related Products

Description Clone Applications

Pathways

  • B cell Development and Activation
  • Cancer Immunoediting
  • Cytokine Network
  • Dendritic Cells
  • Hematopoiesis from Pluripotent Stem Cell
  • IL-10 Pathway
  • IL-6 Pathway
  • Immunologic Networks 2011
  • Innate Immune Signaling
  • Innate Immunity
  • MDSC-induced Immune Suppression
  • Myeloid-Derived Suppressor Cells
  • Neurodegeneration
  • Neuroinflammation
  • NFAT Signaling and Lymphocyte Interactions
  • Regulatory T Cells
  • Stat3-mediated Tumor Escape
  • T Follicular Helper Cell Pathway
  • Th17 Biology

Pages

  • Quality Control
  • Biofunctional Antibodies - LEAF™ and Ultra-LEAF™
  • Interleukin Receptors
  • Chemokines and Chemokine Receptors

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Other Formats

View All IL-6 Reagents Request Custom Conjugation

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 06/19/2019

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Ultra-LEAF Purified anti-human IL-6 Antibody, IL-6, MQ2-13A5 (2024)
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